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full length wildtype human lamp1  (Addgene inc)


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    Addgene inc full length wildtype human lamp1
    (A) Representative time-lapse images showing breakage of the connection between the growing tip of a newly-formed ER tubule (magenta) and a lysosome (green) in EGFP-VAPA(KD/MD)-expressing cells. See table S2 for the compromised contact sites upon VAPA(KD/MD) overexpression. See Movie S5. (B) Average velocities (black spots) of initially ER-tethered lysosomes that become detached (red arrow) from their associated ER growing tips. Detachment events are ER-lysosome connection breakages in EGFP-VAPA(KD/MD)-expressing cells as in (A). **= p<0.01, **** = p<0.0001 (Tukey’s one-way ANOVA). Velocities of 22 events from three independent experiments were quantified. See table S3. (C) Left: Diagram depicting the regions defined as perinuclear and peripheral regions for the following quantification of lysosome distribution, same definition for ( E ). Right: Percentage of the ER comprising tubules upon knockdown of lysosome motion adaptors. Data are shown as ± SEM. **** = p<0.0001 (Tukey’s one-way ANOVA). Data from 20 cells from 3 independent experiments were analyzed for each condition. See table S4. (D) Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with siRNAs. (E) i : Diagram depicting individual components of the chemogenetic system. ii : Quantification of lysosome intensity change and percentage of the ER comprising tubules after 1 hr inducer treatment. Data are shown as ± SEM. **** = p<0.0001 (Student’s t test). For lysosome intensity analysis, N = 10, for ER tubule percentage, N = 20. iii : Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with inducers. (F) Optogenetic assay for repositioning of <t>LAMP1.</t> ( G-J ): Live-cell imaging ( G ), representative zoom-ins ( H ), representative kymograph ( I ) and quantification ( J ) of LAMP1-mCherry-iLID and YFP-SEC61B in COS-7 cells expressing opto-kinesin before or during activation. White arrows indicate lysosome pulling ER tubule (yellow asterisk). Quantification shows mean (± S.E.M.) normalized peripheral SEC61 and LAMP1 intensity of 8 cells. Blue box indicates illumination with blue light. Scale bars represent 1 μm in ( A ) ( G ) ( H ) and ( I ), 5 μm in ( D ) and ( E ). s, seconds.
    Full Length Wildtype Human Lamp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/full+length+wildtype+human+lamp1/bio_rxiv__2020__01__15__907444-198-0-21?v=Addgene+inc
    Average 90 stars, based on 1 article reviews
    full length wildtype human lamp1 - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "The structure and global distribution of the endoplasmic reticulum network is actively regulated by lysosomes"

    Article Title: The structure and global distribution of the endoplasmic reticulum network is actively regulated by lysosomes

    Journal: bioRxiv

    doi: 10.1101/2020.01.15.907444

    (A) Representative time-lapse images showing breakage of the connection between the growing tip of a newly-formed ER tubule (magenta) and a lysosome (green) in EGFP-VAPA(KD/MD)-expressing cells. See table S2 for the compromised contact sites upon VAPA(KD/MD) overexpression. See Movie S5. (B) Average velocities (black spots) of initially ER-tethered lysosomes that become detached (red arrow) from their associated ER growing tips. Detachment events are ER-lysosome connection breakages in EGFP-VAPA(KD/MD)-expressing cells as in (A). **= p<0.01, **** = p<0.0001 (Tukey’s one-way ANOVA). Velocities of 22 events from three independent experiments were quantified. See table S3. (C) Left: Diagram depicting the regions defined as perinuclear and peripheral regions for the following quantification of lysosome distribution, same definition for ( E ). Right: Percentage of the ER comprising tubules upon knockdown of lysosome motion adaptors. Data are shown as ± SEM. **** = p<0.0001 (Tukey’s one-way ANOVA). Data from 20 cells from 3 independent experiments were analyzed for each condition. See table S4. (D) Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with siRNAs. (E) i : Diagram depicting individual components of the chemogenetic system. ii : Quantification of lysosome intensity change and percentage of the ER comprising tubules after 1 hr inducer treatment. Data are shown as ± SEM. **** = p<0.0001 (Student’s t test). For lysosome intensity analysis, N = 10, for ER tubule percentage, N = 20. iii : Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with inducers. (F) Optogenetic assay for repositioning of LAMP1. ( G-J ): Live-cell imaging ( G ), representative zoom-ins ( H ), representative kymograph ( I ) and quantification ( J ) of LAMP1-mCherry-iLID and YFP-SEC61B in COS-7 cells expressing opto-kinesin before or during activation. White arrows indicate lysosome pulling ER tubule (yellow asterisk). Quantification shows mean (± S.E.M.) normalized peripheral SEC61 and LAMP1 intensity of 8 cells. Blue box indicates illumination with blue light. Scale bars represent 1 μm in ( A ) ( G ) ( H ) and ( I ), 5 μm in ( D ) and ( E ). s, seconds.
    Figure Legend Snippet: (A) Representative time-lapse images showing breakage of the connection between the growing tip of a newly-formed ER tubule (magenta) and a lysosome (green) in EGFP-VAPA(KD/MD)-expressing cells. See table S2 for the compromised contact sites upon VAPA(KD/MD) overexpression. See Movie S5. (B) Average velocities (black spots) of initially ER-tethered lysosomes that become detached (red arrow) from their associated ER growing tips. Detachment events are ER-lysosome connection breakages in EGFP-VAPA(KD/MD)-expressing cells as in (A). **= p<0.01, **** = p<0.0001 (Tukey’s one-way ANOVA). Velocities of 22 events from three independent experiments were quantified. See table S3. (C) Left: Diagram depicting the regions defined as perinuclear and peripheral regions for the following quantification of lysosome distribution, same definition for ( E ). Right: Percentage of the ER comprising tubules upon knockdown of lysosome motion adaptors. Data are shown as ± SEM. **** = p<0.0001 (Tukey’s one-way ANOVA). Data from 20 cells from 3 independent experiments were analyzed for each condition. See table S4. (D) Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with siRNAs. (E) i : Diagram depicting individual components of the chemogenetic system. ii : Quantification of lysosome intensity change and percentage of the ER comprising tubules after 1 hr inducer treatment. Data are shown as ± SEM. **** = p<0.0001 (Student’s t test). For lysosome intensity analysis, N = 10, for ER tubule percentage, N = 20. iii : Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with inducers. (F) Optogenetic assay for repositioning of LAMP1. ( G-J ): Live-cell imaging ( G ), representative zoom-ins ( H ), representative kymograph ( I ) and quantification ( J ) of LAMP1-mCherry-iLID and YFP-SEC61B in COS-7 cells expressing opto-kinesin before or during activation. White arrows indicate lysosome pulling ER tubule (yellow asterisk). Quantification shows mean (± S.E.M.) normalized peripheral SEC61 and LAMP1 intensity of 8 cells. Blue box indicates illumination with blue light. Scale bars represent 1 μm in ( A ) ( G ) ( H ) and ( I ), 5 μm in ( D ) and ( E ). s, seconds.

    Techniques Used: Expressing, Over Expression, Knockdown, Control, Live Cell Imaging, Activation Assay



    Similar Products

    full length wildtype human lamp1  (Addgene inc)
    90
    Addgene inc full length wildtype human lamp1
    (A) Representative time-lapse images showing breakage of the connection between the growing tip of a newly-formed ER tubule (magenta) and a lysosome (green) in EGFP-VAPA(KD/MD)-expressing cells. See table S2 for the compromised contact sites upon VAPA(KD/MD) overexpression. See Movie S5. (B) Average velocities (black spots) of initially ER-tethered lysosomes that become detached (red arrow) from their associated ER growing tips. Detachment events are ER-lysosome connection breakages in EGFP-VAPA(KD/MD)-expressing cells as in (A). **= p<0.01, **** = p<0.0001 (Tukey’s one-way ANOVA). Velocities of 22 events from three independent experiments were quantified. See table S3. (C) Left: Diagram depicting the regions defined as perinuclear and peripheral regions for the following quantification of lysosome distribution, same definition for ( E ). Right: Percentage of the ER comprising tubules upon knockdown of lysosome motion adaptors. Data are shown as ± SEM. **** = p<0.0001 (Tukey’s one-way ANOVA). Data from 20 cells from 3 independent experiments were analyzed for each condition. See table S4. (D) Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with siRNAs. (E) i : Diagram depicting individual components of the chemogenetic system. ii : Quantification of lysosome intensity change and percentage of the ER comprising tubules after 1 hr inducer treatment. Data are shown as ± SEM. **** = p<0.0001 (Student’s t test). For lysosome intensity analysis, N = 10, for ER tubule percentage, N = 20. iii : Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with inducers. (F) Optogenetic assay for repositioning of <t>LAMP1.</t> ( G-J ): Live-cell imaging ( G ), representative zoom-ins ( H ), representative kymograph ( I ) and quantification ( J ) of LAMP1-mCherry-iLID and YFP-SEC61B in COS-7 cells expressing opto-kinesin before or during activation. White arrows indicate lysosome pulling ER tubule (yellow asterisk). Quantification shows mean (± S.E.M.) normalized peripheral SEC61 and LAMP1 intensity of 8 cells. Blue box indicates illumination with blue light. Scale bars represent 1 μm in ( A ) ( G ) ( H ) and ( I ), 5 μm in ( D ) and ( E ). s, seconds.
    Full Length Wildtype Human Lamp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/full+length+wildtype+human+lamp1/bio_rxiv__2020__01__15__907444-198-0-21?v=Addgene+inc
    Average 90 stars, based on 1 article reviews
    full length wildtype human lamp1 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Representative time-lapse images showing breakage of the connection between the growing tip of a newly-formed ER tubule (magenta) and a lysosome (green) in EGFP-VAPA(KD/MD)-expressing cells. See table S2 for the compromised contact sites upon VAPA(KD/MD) overexpression. See Movie S5. (B) Average velocities (black spots) of initially ER-tethered lysosomes that become detached (red arrow) from their associated ER growing tips. Detachment events are ER-lysosome connection breakages in EGFP-VAPA(KD/MD)-expressing cells as in (A). **= p<0.01, **** = p<0.0001 (Tukey’s one-way ANOVA). Velocities of 22 events from three independent experiments were quantified. See table S3. (C) Left: Diagram depicting the regions defined as perinuclear and peripheral regions for the following quantification of lysosome distribution, same definition for ( E ). Right: Percentage of the ER comprising tubules upon knockdown of lysosome motion adaptors. Data are shown as ± SEM. **** = p<0.0001 (Tukey’s one-way ANOVA). Data from 20 cells from 3 independent experiments were analyzed for each condition. See table S4. (D) Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with siRNAs. (E) i : Diagram depicting individual components of the chemogenetic system. ii : Quantification of lysosome intensity change and percentage of the ER comprising tubules after 1 hr inducer treatment. Data are shown as ± SEM. **** = p<0.0001 (Student’s t test). For lysosome intensity analysis, N = 10, for ER tubule percentage, N = 20. iii : Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with inducers. (F) Optogenetic assay for repositioning of LAMP1. ( G-J ): Live-cell imaging ( G ), representative zoom-ins ( H ), representative kymograph ( I ) and quantification ( J ) of LAMP1-mCherry-iLID and YFP-SEC61B in COS-7 cells expressing opto-kinesin before or during activation. White arrows indicate lysosome pulling ER tubule (yellow asterisk). Quantification shows mean (± S.E.M.) normalized peripheral SEC61 and LAMP1 intensity of 8 cells. Blue box indicates illumination with blue light. Scale bars represent 1 μm in ( A ) ( G ) ( H ) and ( I ), 5 μm in ( D ) and ( E ). s, seconds.

    Journal: bioRxiv

    Article Title: The structure and global distribution of the endoplasmic reticulum network is actively regulated by lysosomes

    doi: 10.1101/2020.01.15.907444

    Figure Lengend Snippet: (A) Representative time-lapse images showing breakage of the connection between the growing tip of a newly-formed ER tubule (magenta) and a lysosome (green) in EGFP-VAPA(KD/MD)-expressing cells. See table S2 for the compromised contact sites upon VAPA(KD/MD) overexpression. See Movie S5. (B) Average velocities (black spots) of initially ER-tethered lysosomes that become detached (red arrow) from their associated ER growing tips. Detachment events are ER-lysosome connection breakages in EGFP-VAPA(KD/MD)-expressing cells as in (A). **= p<0.01, **** = p<0.0001 (Tukey’s one-way ANOVA). Velocities of 22 events from three independent experiments were quantified. See table S3. (C) Left: Diagram depicting the regions defined as perinuclear and peripheral regions for the following quantification of lysosome distribution, same definition for ( E ). Right: Percentage of the ER comprising tubules upon knockdown of lysosome motion adaptors. Data are shown as ± SEM. **** = p<0.0001 (Tukey’s one-way ANOVA). Data from 20 cells from 3 independent experiments were analyzed for each condition. See table S4. (D) Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with siRNAs. (E) i : Diagram depicting individual components of the chemogenetic system. ii : Quantification of lysosome intensity change and percentage of the ER comprising tubules after 1 hr inducer treatment. Data are shown as ± SEM. **** = p<0.0001 (Student’s t test). For lysosome intensity analysis, N = 10, for ER tubule percentage, N = 20. iii : Representative images showing the distribution of lysosomes and ER tubules in control cells and in cells treated with inducers. (F) Optogenetic assay for repositioning of LAMP1. ( G-J ): Live-cell imaging ( G ), representative zoom-ins ( H ), representative kymograph ( I ) and quantification ( J ) of LAMP1-mCherry-iLID and YFP-SEC61B in COS-7 cells expressing opto-kinesin before or during activation. White arrows indicate lysosome pulling ER tubule (yellow asterisk). Quantification shows mean (± S.E.M.) normalized peripheral SEC61 and LAMP1 intensity of 8 cells. Blue box indicates illumination with blue light. Scale bars represent 1 μm in ( A ) ( G ) ( H ) and ( I ), 5 μm in ( D ) and ( E ). s, seconds.

    Article Snippet: Full length wildtype human LAMP1, was derived from LAMP1-mGFP , a gift from Esteban Dell’Angelica (University of California, Los Angeles, CA; Addgene plasmid #34831).

    Techniques: Expressing, Over Expression, Knockdown, Control, Live Cell Imaging, Activation Assay